During 2003, 4 cruises were conducted with RV 'Luctor' in January, April, July and October, 1 in each of 4 seasons. Five stations were sampled during every cruise. The stations were of fixed salinity but their exact location varied depending on tide and discharge.
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The salinities of the 5 stations were 0, 2, 8, 18 and 28, and these numbers are used as station names from this point onwards. Station 0 was located close to Dendermonde, Belgium, 122 km from the mouth of the estuary, whereas Station 28 was located at the mouth of the estuary, close to Vlissingen, the Netherlands.
Water was sampled in 20 L Niskin bottles from approximately 2 m depth and subsampled immediately after retrieval of each bottle.
