Algal-bacterial coupling in the Western Scheldt (The Netherlands) assessed through isotope labeling [Koppeling van primaire en bacteriële produktie in de Westerschelde: experiment met labeling met stabiele isotopen]

Citation

Van den Meersche K., Soetaert K. 2004: Algal-bacterial coupling in the Western Scheldt (The Netherlands) assessed through isotope labeling. Netherlands Institute of Ecology; Centre for Estuarine and Marine Ecology, Netherlands. Metadata available at http://mda.nioo.knaw.nl/imis.php?module=dataset&dasid=1224. https://marineinfo.org/id/dataset/1224

Archived data

Archived file:

labellingsexperiment 06-04.mdb

Availability: Unrestricted
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Description

Carbon flows in a natural phytoplankton assemblage from the Western Scheldt estuary were followed by measuring changes in concentrations and stable isotope ratios in a 10-day period after addition of 13C to a batch experiment. more

Water was collected from the euhaline reaches of the estuary, near Vlissingen, and incubated in a mesocosm setup. A phytoplankton bloom was initiated and followed up through the subsequent collapse. Coupling of algal and bacterial production was estimated from 13C-uptake in protist- and bacterial-specific polar lipid fatty acids (PLFA) with aid of a dynamic model. As expected, algal exudates turned out to be the dominant source for bacterial carbon production, showing a strong microbial loop. 60% of bacterial carbon production could be attributed to regenerated in situ primary production. Zooplankton showed a preference for phytoplankton material, but was able to switch to other food sources when the algal bloom collapsed.

Scope

Themes:

Biology, Biology > Benthos, Biology > Invertebrates, Biology > Plants

Keywords:

Brackish water, Algae, Bacteria, Carbon 13, Isotopic labelling, Microbial loop, ANE, Netherlands, Westerschelde, , Bacteria, Copepoda

Geographical coverage

ANE, Netherlands, Westerschelde [Marine Regions]

Temporal coverage

4 June 2004 - 14 June 2004

Taxonomic coverage

Algae

Bacteria [WoRMS]

Copepoda [WoRMS]

Parameters

Alkalinity (pH)
Ammonium (NH₄⁺)
Bicarbonate (HCO3-)
Carbon (C) per mesozooplankton
Count of mesozooplankton
Dissolved organic carbon (DOC)
Dissolved oxygen % saturation
Free phosphate concentration (PO43-)
Isotope %15N-NH4
Isotope 13C in phospholipid fatty acid
Isotope 13C-DIC from filtrated water
Isotope 13C-DOC from filtrated water
Isotope Carbon (C)
Isotope Nitrogen (N)
Isotope N-NH4o/oo
Isotope N-NO3o/oo
Microzooplankton
Molar ratio C:N
Nitrate (NO₃^-)
Nitrate (NO3-) + Nitrite (NO2-)
Nitrite (NO₂^-)
Nitrogen (N) per mesozooplankton
Organic carbon (OC)
Phospholipid-derived fatty acids (PLFAs)
Phytoplankton Methodology
Pigment concentration
Salinity (titrimetric with silver nitrate)
Silicate (SiO₄^4-)
Temperature in water
Total nitrogen (N)
Urea in seawater
Zooplankton isotopic signature 13C
Zooplankton isotopic signature 15N

Phytoplankton: Microscopic cell count

Dataset status: In Progress

Data type: Data

Data origin: Research: lab experiment

Metadatarecord created: 2007-04-18

Information last updated: 2009-10-08

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